AURAMINE

Stains Indicator Reagents-69

AURAMINE

Auramine O is Basic yellow 2 is a diarylmethane dye used as a fluorescent stain.

Key details

Standard packing 100.00 Grams
Available pack sizes AURAMINE laboratory grade available in 50, 100, 200 Gram packing. While commercial grade of Auramine O is available in H.D.P.E bags 50 Kg PACKING.
Supply locations AURAMINE manufacturer supplier distributor in Mumbai, Kandla, Kolkata, Vizag, Chennai, India, Fujairah, Dubai, Sharjah, UAE, Gulf, Middle East, Muscat Oman, Kenya Africa. Get the best quality of AURAMINE at a competitive price from us. We have ready stock of AURAMINE in India, UAE Gulf, Oman, Kenya Africa. Contact us for bulk as well as small orders.

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Application

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta. Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Using Procedure

Auramine O is Basic yellow 2 is a diarylmethane dye used as a fluorescent stain. In its pure form, Auramine O appears as yellow needle crystals.
Auramine O can be used to stain acid-fast bacteria (e.g. Mycobacterium), where it binds to the mycolic acid in its cell wall) in a way similar to Ziehl-Neelsen stain. It can also be used as a fluorescent version of Schiff reagent.

Auramine O can be used together with Rhodamine B as the Truant auramine-rhodamine stain for Mycobacterium tuberculosis. It can be also used as an antiseptic agent.

Technical Specifications

Specifications:

 

MOLECULAR FORMULA    C17H21N3·HCl 
MOLECULAR WEIGHT   303.83
COLOUR CRUDE YELLOW POWDER
DYE CONTENT 85 %
ABSORPTION MAXIMUM λ (ph 6.0) 431-433 nm
ABSORPTIVITY (A 1%/1cm λ MAX ph 6.0 ON DRIED MATERIAL  850-1000
SUITABILITY FOR MICROSCOPY PASSES TEST
LOSS ON DRYING AT 1100 C MAX 2 %
Remarks

Handling and Storage
Keep in a tightly closed container, stored in a cool, dry, ventilated area. Protect against physical damage. Separate from incompatibilities. Containers of this material may be hazardous when empty since they retain product residues (dust, solids); observe all warnings and precautions listed for the product.

Note

Commercially Auramine O can be used in the dyeing of acrylic fibres, silk and cotton fiber; also applied in the dyeing of leather, paper, hemp and viscose; Auramine O can also be used in the direct printing and blotch discharge printing of tannin mordant dyed cotton, and also used to produce colour lake and used in inks.

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