AZURE A

Stains Indicator Reagents-69

AZURE A

Azure A helps for Nucleolus Staining

Dark Greenish Black Powder soluble in water.

Key details

Generic name Azure A helps for Nucleolus Staining
Active matter (%) 70 - 80
Standard packing 1.00 No
Available pack sizes AZURE A is Dark Greenish Black Powder. Analytical - Lab Reagent available in 5, 10, 50, 100, 500, 1 Kg Packing.
Supply locations AZURE A supplier in Mumbai, Surat, Gandhidham, Chennai, Visakahapatnam, Kolkata, Fujairah, Dubai, Sharjah.

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Application

<p style="text-align: justify;">
<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;">Azure A is a basic cationic dye.  The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. It is a main component of the azure B/polychromed methylene blue – eosin stains family, also referred to as the Romanowsky stain.</span></p>

Using Procedure

<p style="text-align: justify;">
AZURE A is Dark Greenish Black  Powder. When it mix with 0.1% DM / DI water resulting CLEAR BLUE SOLUTION. AZURE A is a<span style="font-family: Arial, Helvetica, sans-serif; font-size: small;">n important nuclear staining component of polychrome methylene blue. For use in Lillie's modified Nocht's method for paraffin sections and the NCCLS method for blood smears. </span><span style="font-family: Arial, Helvetica, sans-serif; font-size: small;">It is used in making azure eosin stains for blood smear staining. It has a role as a histological dye and a fluorochrome.</span></p>

Technical Specifications
C.A.S. No. 531-53-3
CI NO. 52005
M.F. C14H14CIN3S
M.W. 291.80gm/mole

Specifications:

Appearance Powder
Colour Blue
Dye Content (by Spectrophotometry) >75%
Solubility 0.1% (Dist. Water) Clear solution
Absorption Maximum (Dist.Water) ?max 628-633nm
Absorptivity (A1%, 1cm in Water, at ?max on dried substance) >1120
Loss On Drying (110°C) <8%
Suitability for microscopy Passes test
Remarks

<p>
<span style="color: rgb(102, 94, 88); font-family: Arial, Helvetica, sans-serif;">Handle and store under argon. Glove bag item.</span></p>

Note

<p>
<span style="text-align: justify; font-family: Arial, Helvetica, sans-serif; font-size: small;">CAS Number:531-53-3</span><br style="text-align: justify; font-family: Arial, Helvetica, sans-serif;" />
<span style="text-align: justify; font-family: Arial, Helvetica, sans-serif; font-size: small;">Molecular Weight:291.80</span><br style="text-align: justify; font-family: Arial, Helvetica, sans-serif;" />
<span style="text-align: justify; font-family: Arial, Helvetica, sans-serif; font-size: small;">Molecular Formula:C14H14Cl N3S</span></p>

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