BIEBRICH SCARLET

Stains Indicator Reagents-69

BIEBRICH SCARLET

Acid Red 66

Biebrich scarlet: an acid azo dye that is used as a biological stain for cytoplasm. Fluorescent labeling of white cells in tissue samples

Key details

Generic name Acid Red 66
Standard packing 1.00 No
Available pack sizes Biebrich Scarlet for Fluorescent Staining of Cells.
Supply locations Keeping ready stock at Gandhidham, Mumbai, Chennai, Visakhapatnam, Kolkata, Fujairah, Dubai, Sharjah, Abu Dhabi

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Application

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<div style="text-align: justify;">
APPLICATION: FOR MICROSCOPICAL STAINING <br />
Biebrich scarlet: an acid azo dye that is used as a biological stain for cytoplasm.<br />
Fluorescent labeling of white cells in tissue samples<br />
The stain, Biebrich scarlet, is photo fixing and is especially useful for identifying eosinophils by binding to their cytoplasmic granules in tissue samples from 6 to 60 microns in thickness. It also stains granules of macrophage and granular lymphocytes.</div>
</div>

Using Procedure

<p>
<span style="text-align: justify;">BIEBRICH SCARLET is </span>Soluble in water, ethanol</p>

Technical Specifications
C.A.S. No. 4196-99-0
CI NO. 26905
M.F. C22H14N4Na2O7S2
M.W. 556.49gm/mole

Specifications:

 

Dye Content (by Spectrophotometry) >70%
Solubility 0.1% (Dist. Water) Clear solution
Absorption Maximum (Dist. Water) ?max 506-510nm
Absorptivity (A1%/1cm,in water at ?max on dried substance) 530-740
Loss On Drying (110°C) <15%
Suitability for microscopy Passes test
Note

Appearance : Red to brown powder

Solubility : 20 ppm in methanol yields clear solution

Absorption maxima : 500 - 510 nm (20 ppm in methanol)

Loss on drying (at 110°C, 1hr) : <= 15.0%

Appearance : Red to brown powder

 

Solubility : 20 ppm in methanol yields clear solution

 

Absorption maxima : 500 - 510 nm (20 ppm in methanol)

 

Loss on drying (at 110°C, 1hr) : <= 15.0%

 

Synonym  : Ponceau BS; Acid Red 66 

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