NNNN TETRAMETHYL p PHENYLENEDIAMINE DIHYDROCHLORIDE

Lab Chemical-60

NNNN TETRAMETHYL p PHENYLENEDIAMINE DIHYDROCHLORIDE

Part No. RXSOL-60-6605-022

NNNN TETRAMETHYL p PHENYLENEDIAMINE DIHYDROCHLORIDE

Key details

Part number RXSOL-60-6605-022
Standard packing 0.00 Box
Supply locations NNNN TETRAMETHYL p PHENYLENEDIAMINE DIHYDROCHLORIDE manufacturers, suppliers, exporters in Mumbai, Gandhidham, Kolkata, Varanasi, Visakhapatnam, Chennai, Fujairah, Dubai, Canada BC, Barka, Sohar, Muscat, Oman. Lab chemicals manufacturers, suppliers, exporters in India, UAE Middle East, Barka, Sohar, Muscat, Oman, Canada. NNNN TETRAMETHYL p PHENYLENEDIAMINE DIHYDROCHLORIDE is available in small packing as well as in bulk. Buy premium quality NNNN TETRAMETHYL p PHENYLENEDIAMINE DIHYDROCHLORIDE and other lab chemicals from one of the most trusted brands.

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Application

<p>
<span style="color:#000;"><strong><em><span style="font-family:georgia,serif;"><span style="font-size:14px;">Kovacs reagent is a biochemical reagent consisting of </span></span></em></strong></span><strong><em><span style="font-family:georgia,serif;"><span style="font-size:14px;"><a href="https://en.wikipedia.org/wiki/Isoamyl_alcohol&quot; title="Isoamyl alcohol"><span style="color:#000;">isoamyl alcohol</span></a><span style="color:#000;">, </span><a href="https://en.wikipedia.org/wiki/Para-dimethylaminobenzaldehyde&quot; title="Para-dimethylaminobenzaldehyde"><span style="color:#000;">para-dimethylaminobenzaldehyde</span></a><span style="color:#000;"> (DMAB), and concentrated </span><a href="https://en.wikipedia.org/wiki/Hydrochloric_acid&quot; title="Hydrochloric acid"><span style="color:#000;">hydrochloric acid</span></a><span style="color:#000;">. It is used for the diagnostical </span><a href="https://en.wikipedia.org/wiki/Indole_test&quot; title="Indole test"><span style="color:#000;">indole test</span></a><span style="color:#000;">, to determine the ability of the organism to split </span><a href="https://en.wikipedia.org/wiki/Indole&quot; title="Indole"><span style="color:#000;">indole</span></a><span style="color:#000;"> from the </span><a href="https://en.wikipedia.org/wiki/Amino_acid&quot; title="Amino acid"><span style="color:#000;">amino acid</span></a><span style="color:#000;"> </span><a href="https://en.wikipedia.org/wiki/Tryptophan&quot; title="Tryptophan"><span style="color:#000;">tryptophan</span></a><span style="color:#000;">. The indole produced yields a red complex with para-dimethylaminobenzaldehyde under the given conditions.</span><a href="https://en.wikipedia.org/wiki/Kovac's_reagent#cite_note-Siegrist-1"><sp… style="color:#000;">[1]</span></a></span></span></em></strong><span style="color:#000;"><strong><em><span style="font-family:georgia,serif;"><span style="font-size:14px;"> This was invented by the Hungarian-Swiss Chemist, Ervin Kovats (Erwin Kovacs) (1927–2010). This reagent is used in the confirmation of E. coli and many other pathogenic microorganisms.</span></span></em></strong></span></p>

Using Procedure

<p>
<strong><em><span style="font-family:georgia,serif;"><span style="font-size:14px;">Wurster's blue is the trivial name given to the chemical N,N,N′,N′-tetramethyl-p-phenylenediamine, also known as TMPD. It is an easily oxidised <a href="https://en.wikipedia.org/wiki/Phenylenediamine&quot; title="Phenylenediamine">phenylenediamine</a>, which loses two <a href="https://en.wikipedia.org/wiki/Electron&quot; title="Electron">electrons</a> in one-electron <a href="https://en.wikipedia.org/wiki/Oxidation&quot; title="Oxidation">oxidation</a> steps; the <a href="https://en.wikipedia.org/wiki/Radical_cation&quot; title="">radical cation</a> is a characteristic blue-violet colour, which gives the compound part of its name. The remaining part of its name comes from its discoverer, the German <a href="https://en.wikipedia.org/wiki/Chemist&quot; title="Chemist">chemist</a> Casimir Wurster </span></span></em></strong></p>

Note

<p>
<em><strong><span style="font-family:georgia,serif;"><span style="font-size:14px;">The term "Wurster's blue" is often reserved for the radical cation, the colorless diamine being called tetramethylphenylenediamine (TMPD). The midpoint potential for titration of the first electron is given as 0.276 V vs NHE, and this transition is useful in potentiometric titrations as both a redox mediator and indicator. The two electron-oxidized form (di-iminium) is unstable in aqueous solutions,<a href="https://en.wikipedia.org/wiki/Wurster's_blue#cite_note-1">[1]</a&gt; therefore highly oxidizing conditions should be avoided in titrations relying on TMPD, or reached only during the final stage of the titration. </span></span></strong></em></p>

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